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by Kirk N.
NOTE: I am a man of conscience and I
would NEVER do anything
to purposefully violate ANY laws.
Everything contained herein
is SOLEY a product of an active
imagination - and EVERYTHING
is TRULY hypothetical. So any
resemblance to methods which are
seemingly realistic is PURELY
coincidental.
EXPERIMENT: Attempt to grow shrooms.
METHODS TRIED:
- MMGG
- Home-Brew soil method.
Method #1:
Status:
MMGG has failed tragically due to
error on my part which led
to contamination. Contamination
appears as white dots, and there is a
putrid odor emanating from the
holes in the jars.
Possible causes:
- Too much water.
- Mason jars sterilized in boiling water for only 45 minutes.
- Room too dusty; a product of my all-around lazy attitude toward cleaning.
Method #2:
Status:
This method has proven successful,
as now pinning is apparent.
HOW TO SUCCED WITH SOIL METHOD:
Simplified instructions.
NEEDED:
- 9" diameter round Tupperware (tm)
plastic food-saver pot-like thingy,
which comes with lid. Should be 4.5"
deep.
- Rich potting soil, preferribly
containing sterilized cow-maneur.
- Vermiculite -- $2.79 (2-gallon bag) at
K-Mart. If you go to their
garden department, they won't have any
and they'll tell you they
don't sell it. They do -- they LIE -- but
NOT in the garden section.
- PF spore syringe -- $10.00.
- Coffee beans. I have a coffee tree
growing in my house, so it was
EASY to acquire them. Unfortunately,
grocery-store beans are NOT
good because they have junk in them and
have been roasted BLACK and
have NO shells on them. The fungus
seems to like the shells MORE than
the nut inside, which is whitish. I tried
other types of beans,
berries and seeds - and NONE worked.
They needed too much moisture,
and of also needed to be soft ans
sterilized for the fungus to have
a shot at infecting it -- the very same
conditions that favor
bacterial growth. The coffee beans work
miraculously well and DO NOT
NEED TO BE STERILIZED. The soil is
forgiving, as it retards infective
outbreaks. Hey, it worked for me. If
nobody can find coffee beans, and
I manage to MASTER my techniques so
that they can be consistently
reproducible, then perhaps I'll consider
sending people coffee beans.
I BUY my coffee -- when I want coffee --
and I don't have a coffee
grinder, so the beans are otherwise of no
use to me. Unlike Juan Valdez,
I have better things to do than to open
shells and roast coffee beans for
a lousy cup of coffee.
Coffee beans are good because they don't
dessicate quickly and
always seem to be moist. In fact, when
you place them on the
soil dry and cracked, the shells seem to
draw moisture from the
soil, providing a nice medium for the
fungus. You can leave the
beans out normally for seeming eons,
and no infection appears.
Place them on soil that has been sprayed
with spores, and they
show fuzz in a couple days.
- Fresh sheet of copy-paper.
TYPE OF CLEANLINESS NEEDED:
Clean room: Nope, don't worry about it.
Sterility of envronment: Nope, don't
worry about it.
Sterility of utensils touching soil: Nope,
don't worry about it.
Sterility of soil: If you purchase sterilized
potting soil and
sterilized maneur, that is ENOUGH.
Don't worry about it. If the
stuff ISN'T sterilized, place tupperware in
microwave and
heat that sucker up until its really hot.
Then take it out, let
it cool, and STIR ALOT!
PROCEDURES: THE GOOD PART
- Fill container with soil mixture and
make sure mixture REALLY IS
a mixture. If not, OR if you made the soil
solution yourself, then
STIR THOROUGHLY. Add SOME (you
decide, I used 1 cup) vermiculite,
and stir.
- Cut, drill, or force (4) NAIL-HOLES
into the SIDE of the tupperware
container .75 (3/4) INCH BELOW the
surface of the soil, ON THE SIDE OF
THE CONTAINER -- EQUALLY SPACED
APART. This is for adding MOISTURE TO
THE SOIL WITHOUT WATERING THE
SURFACE, with the aid of the USED
SPORE
SYRINGE.
- Cut coffee beans in HALF, but leave
shells on. Place them into a
cup of water for 5 minutes, then remove
and place on surface of soil,
halfway inserted, pointed up (beans ~
ovular shaped)
- Take CLEAN cup, fill with vermiculite
and HOT water. Allow soaking,
and then remove the vermiculite and
wring out partially with aid of
paper towel. Scatter small quantity of
vermiculite on surface of soil.
You don't want to COVER the soil
completely, but just patchy. It holds
moisture and prevents dessication of soil
surface.
- Water soil LIGHTLY. You *DON'T*
want the surface to be TOO moist,
or you MAY have contamination.
Contamination will EITHER appear
as white spots, OR as tiny greenish dots.
The healthy mycelium is
a thick white fuzzy thing. If
contamination occurs, consider your
project a failure. I tried this several ways,
and only ONE way
became contaminated; I used a lot of
moisture, and placed it in
ABSOLUTE darkness with LID on for 2
days. Tiny green spots appeared
UNDER the white fuzz. So at early stages,
mycelium WILL NOT fight
off infection. I don't know WHAT this
green stuff was, but it moved
in FAST.
- Spray surface of soil with 3 CCs of
spore solution -- plenty.
- Place PAPER sheet OVER surface of pot
(plastic) and put NEAR
radiator. DO NOT -- I REPEAT -- DO NOT
COVER TUPPEWARE with COVER,
or an infection is ASSURED! Max heat =
85 degrees F. Covering with
LID is beneficial for one-hour periods
DAILY. But if you leave it on,
it will become infected. You can and
SHOULD always leave the paper
on. Don't ask me why; I did several pots
and I'm telling you what
works. The key reason why this works is
that the fungus, unlike
bacteria, seems to have NO PROBLEM
invading the soil quickly
without a lot of moisture. Of course the
fungi would like a little
more, but better safe than sorry. If you
keep the moisture level
LOW, then you will most likely succeed.
Once it raises above a
critical level, there goes the
neighborhood!
- Daily, remove paper and inject small
quantities of CLEAN water
UNDER surface of soil. You can, as I did,
water the surface VERY
lightly with the syringe. But be careful,
or you will damage the
mycelium. You NEVER -- I REPEAT --
NEVER want the SURFACE to SEEM
WET, or even MOIST. It doesn't NEED to
be really moist for the
fungus to spread like MAD. Think DRY,
as it is BETTER too dry than
too moist. As long as there is PLENTY of
oxygen, a VERY small amount
of moisture is PLENTY at the beginning
stages.
- Withing ONE WEEK, the surface
should be NICELY covered with
mycelium.
The heaviest patches will be AROUND
the beans. The first areas to colonize
will be the beans, about 2-3 days after
spraying. Stir the soil up
around the surface. This will SEEM to
destroy the mycelium, and
when you are finished, there will be NO
MORE WHITENESS on the
surface. Don't fret, it will shortly grow
back. After stirring; water,
scatter a little moist vermiculite, and
place the paper back.
- Within another 8-12 days, as long as
the container is BESIDE
the radiator, the fungus will
COMPLETELY take over the surface of
the
soil again (VERY THICK). When it does,
and when it is REALLY THICK,
you can take the pot and place it into a
terrarium like the one
described in MMGG. Make sure it is
WARM (85 DegF), and make SURE it
is HUMID. Case it with moist (wet and
wring out) vermiculite and set
in dark for 4-5 days, before turning on
the aeration and light. Within
a week, PINNING will start.
DISCLAIMER: I have DONE EXACTLY as
outlined, and it WORKED for me, up
to the point of pinning (current=4
pinheads, 2 larger mushies). I
don't know what will happen within the
next week and I have not
consumed anything yet I can only assume
that since people pick
these things (shrooms) off of cow
pastures, and warm, swampy soils,
and consume them, that these are
likewise edible. I don't know, I
should in a week. Injest at your own risk.
Oh yea, use a NICE light
for fruiting. I did, and it works.
I am a student, and I am a gardner. So all
of my experience has come
from growing plants. Fungus is a new
area for me, so I don't know
how or why anything I have done has
worked. I knew full well that
P. Cubensis could be found in cow-
pastures, on the dung or on the
soil - but always in SUNNY places. So I
figured that it would be
worth the experiment. I read a few books,
and found that many
contradicted the others. Then I read many
grow-guides, and decided
I'd try something new. So I did, and this
is it.
The key advantage to my method is that
you do not need a sterile
environment; the soil is VERY forgiving.
If you try the other
methods, YOU MUST -- I REPEAT --
MUST guarantee sterility, or
you will UNDOUBTEDLY FAIL. Even
after the fungus has partially
colonized, bacteria will have NO
PROBLEM RACING IN and taking
over immediately! Many people have
succeeded in doing it, but
many have also failed.
A good method of testing out your
sterilization techniques:
- Take jar w/ substrate and sterilize it.
- Remove it and place it somewhere.
- Wait a week, open and smell. If it
smells funky (putrid), then
you would have failed.
Created 9/26/2000 3:29:40 Modified 9/26/2000 3:31:42 | Leda version 1.4.3 |
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