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[The Lycaeum Forums]

 The Lycaeum Forums » Integrate » The Alchemist's Den (Moderator: tryp2fun)Topic:
 Pine tree DNA extraction help help sos

[daytripper1984]

i am taking a biosynthetic pathway from a plant and trying to insert it into algae.  I have a long way to go till i can make it work but i think its feasible.  do you think a mammalian flu virus would work on algae as well?  it is a plant but its more aquatic than most plants

[whateveryouwantmetobe]

You would be better off trying to work with yeast or E. coli as there are established and readily available (online) protocols for this sort of thing with them.  I doubt you could do much with plants without having high quality academic lab experience practicing this sort of thing.  Really you probably need it period to do any sort of genetic work like this, but at least with the two I mentioned the protocols can be obtained at little or no cost for you to read about.  Then pending what you want to do, there's the whole equipment thing...  you ever do PCR?

[daytripper1984]

Im going to take genes from a tree and insert them into a strain of algae.  I need to find a virus that is host specific for algae.  Also need to break down the pathway in the algae and determine how to alter it to make other products.