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Bacon, Charles W.
Procedure for isolating the endophyte from tall fescue and screening isolates for ergot alkaloids
Applied and Environmental Microbiology v54 p2615-18 November 1988
SUBJECTS: Ergot Fescue Alkaloids Acremonium

Discover, December 1992
Plants - Oh, Wilbur
Stipa robusta, "Sleepy Grass"
to its friends, is a tough plant. Not only does it survive in the rugged terrain of the southwestern Rocky Mountains, but it has also evolved a unique defense against animals that graze on its feathery plumes. It harbors a fungus called Acremonium, which produces a powerful poison that can knock a horse cold for up to a week. The fungus gets passed on to future generations through the plant's seeds. "The fungus gets a home and gets fed, and the grass gets protection from critters that want to eat it," says Indiana University biologist Keith Clay. "So it's a mutually beneficial association, not a disease." In pastures where every other type of vegetation has been nibbled to the ground, one can easily spot the sleepy grass - tall, proud, and untouched.
Clay and his co-workers Richard Petroski and Richard Powell from the US Department of Agriculture have now isolated the chemical that gives the sleepy grass fungus its potent punch. It is an alkaloid called lysergic acid amide. Alkaloids are the poisons in hundreds of poisonous plants, and lysergic acid amide has been found before in a few of them, but never in such high concentrations as in sleepy grass.
Lysergic acid amide is a potent sedative in humans as well; Central American Indians are said to quiet crying infants by feeding them a single sleepy-grass seed. In fact, in the 1950s American pharmaceutical manufacturers (who didn't know about the sleepy grass connection) considered marketing the compound as a prescription sleeping aid. Bu the idea ran aground on a public relations problem. Lysergic Acid amide has a close chemical relative called lysergic acid diethylamide, which is more commonly known as LSD. "When the pharmaceutical industry discovered the compound's link to LSD and all the problems associated with that," says Clay, "they essentially dropped it."


CLAY, KEITH
"Trespassers Will Be Poisoned"
Natural History, September 1989
[fragments of article from sketchy hand-written notes]
Tall Fescue Grass, Kentucky-31
from farm of W.M.Suiter in Menifee County, KY in the 1930s
Cattle develop syndrome resembling ergotism
in mid 1970s fungus in fescue discovered at University of Georgia.
Ascomycete fungus: Acremonium coenophialum
network of hyphae in intercellular spaces
mutualistic endophyte
non-infectious.
Fungus reproduces vegetatively, inhabits grass seeds. cannot produce spores.
Hundreds of other grasses known to harbor similar fungi.
Grasses are otherwise remarkably free of toxic alkaliods.
Grass related to 'sleepy grass' in Kulu valley in India.
Claviceps purpurea, ignis sacer "holy fire".
"...native people in the Amazon use an endophyte-infected sedge for obstetric purposes and in magical/religious ceremonies, the effects of which suggest the presence of ergot alkaloids."
"after discovering the endophyte growing inside toxic tall fescue ... scientists were able to grow it in pure cultures in the laboratory and show that it produced ergot alkaloids"
Keith Clay is assistant professor of biology at Indiana University, Bloomington.


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