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AMES RW
The Influence of Temperature on Mycelial Growth of Psilocybe cubensis, Panaeolus, and Copelandia.
Mycopath. et Mycol. Appl. 9:268-274 (1958)
Temperature. Psilocybin Mushrooms

BADHAM, EDMOND R
The influence of humidity upon transpiration and growth in Psilocybe cubensis
Mycologia; Vol 77(6) 1985 p 932-939
... Materials and Methods -- The strain of P. cubensis used was (ATCC 36462). Cultures were grown on sterilized brown rice (6g/15 ml deionized water) in 60x15 mm Petri dishes until mycelia covered the substrate. The mycelial mats (and substrate) were then removed and placed in 130 mm pie tins with vermiculite, 30ml of deionized water was added, and the containers were covered with glass. All mushrooms were allowed to develop to at least 10mm in height under deep Petri plates (80x100mm) and 12 hour photoperiod of 11.2 W/mý (2685 lux) 'cool white' fluorescent light prior to experimentation. The deep Petri plate was removed (to allow for aeration) when the cultures were weighed daily, and any loss in weight was made up with distilled water. Dry weights were determined to the nearest mg after drying in an oven at 60 degrees C for 24 hours. In each test culture only the largest basidiocarp of the first flush was used for experiments. The others were removed at the beginning of the test period and the water content of the second largest (second rank) was determined at that time to be used as an indication of the initial per cent water of the experimental mushroom (first rank). The water content of these two mushrooms from the same mycelium would be very similar because their size, age, and stage of development was nearly the same and they had been exposed to the same environmental conditions.

BADHAM, EDMOND R
The influence of humidity upon transpiration and growth in Psilocybe cubensis
Mycologia; Vol 77(6) 1985 p 932-939
The influence of humidity upon individual basidiocarps of Psilocybe cubensis was studied using an environmentally controlled wind tunnel and a computer program which helped to model growth and development. Regression models were developed which were able to explain 77% of the variation in the transpiration rate and 68% of the variation in growth rate. Transpiration and growth of this mushroom were significantly correlated with the humidity of the air. The fastest growth and the lowest transpiration occurred at the highest humidities. No inhibition of growth was detected at 0 pascals VPD (100% RH). Misting accelerated growth and transpiration while light had no effect. Although humidity was a very important factor influencing transpiration and growth, the size and shape of the mushroom were also important in water relations. The final water content of basidiocarps with thin stipes or those with larger area-to-volume ratios was significantly lower than that of thick-stiped mushrooms or those small area-to-volume ratios with even when grown under equal humidity. Growth rates under conditions which promoted the highest levels of hydration of the basidiocarp were rapid (up to estimated 4% increase in dry weight per hour).

BADHAM, EDMOND R
Tropisms in the mushroom Psilocybe cubensis.
Mycologia; Vol. 74 (1982) p 275-?
The growth of the mushroom Psilocybe cubensis was studied in a wind tunnel under controlled conditions of wind velocity, humidity, temperature,and light. The basidiocarp stipe grew into the wind up to the time of spore formation. When rotated with the long axis of the stipe perpendicular to the wind, fruitbodies grew upright. When spores began to be formed a negative geotropic curvature of the stipe occurred but no recurvation occurred in a sporeless mutant.

BIGWOOD J; BEUG M
Variation of psilocybin and psilocin levels with repeated flushes (harvests) of mature sporocarps of Psilocybe cubensis (Earle) Singer.
J Ethnopharmacol. 1982 May. 5(3). P 287-91.
Analysis of Psilocybe cubensis (Earle) Singer grown in controlled culture showed that the level of psilocin was generally zero in the first (or sometimes even the second) fruiting of the mushroom from a given culture and that the level reached a maximum by the fourth flush. The level of psilocybin, which was nearly always at least twice the level of psilocin, showed no upward or downward trend as fruiting progressed, but was variable over a factor of four. Samples obtained from outside sources had psilocybin levels varying by over a factor of ten from one collection to the next.

OSS O T; OERIC O N; [MCKENNA, DENNIS & TERENCE]
Psilocybin: Magic Mushroom Grower's Guide.
Psilocybin: Magic Mushroom Grower's Guide; 1976 Berkeley: And/Or Press.
Procedure for growing Psilocybe cubensis mushrooms from spores using agar, rye grain, and sterile kitchen techniques. Discussion of history and lifecycle of mushrooms.

OSS O T; OERIC O N; [MCKENNA, TERENCE & DENNIS]
[mushrooms in India]
Psilocybin: Magic mushroom grower's guide
1984: Heterodox Bengali Hindus announce identification of the Vedic intoxicant Soma as a psilocybian mushroom, Stropharia cubensis. A reform of Hinduism centered around rediscovery of the 6000-year-old Soma rite is begun.

POLLOCK SH
Magic Mushroom Cultivation (1)
Magic Mushroom Cultivation. Herbal Medicine Research Foundation. ISBN 0-930074-01-7
THE RICE-CAKE TECHNIQUE: This technique is extremely easy and highly recommended for its convenience in growing Psilocybe cubensis mushrooms. All that is needed is a pressure cooker, some canning jars, uncontaminated live mushroom starter (mycelia), and brown rice. Either long grain or short grain brown rice may be used. The former is usually more economical. Do not use white rice. It is inferior in quality to brown rice because most of the vitamins have been lost in converting brown to white rice. Into each quart jar place 1/4 cup brown rice and between 1/3 to 1/2 cup tap water. One-half cup or more of water is too much because the rice will turn into mush rather than a cake. One-third cup water leads to a dry cake that is adequate, but mycelia grow faster on the wetter cakes resulting from use of more than 1/3 cup of water. Up to 1/8 teaspoon of agricultural gypsum (calcium sulphate) may be added to each jar prior to sterilization to serve as a buffer, but gypsum is not really necessary. Some cubensis strains seem to prefer it, but so do many contaminants. It seems more practical not to bother using gypsum except for purposes of experimentation to find out if a particular strain will fruit more aggressively with it. In most cases it probably will not make any difference. Invert the dome of each two-piece lid and place it on the mouth of each canning jar with the rubber seal facing upward. Then loosely screw on the lid bands. Pressure cook the jars at 15 lbs. pressure for an hour. Actually 45 minutes at 15 lbs. pressure is adequate, but an hour gives even greater likelihood of complete sterilization. Allow the pressure cooker to cool and remove the jars, screwing the bands tighter until ready to inoculate the rice-cakes with mushroom mycelia. Using a flame-sterilized probe, carefully transfer a piece of agar medium containing live uncontaminated mycelia into each jar. It is best to loosen the jar lid beforehand so that it will lift off easily. To make the transfer, cut out a section of the agar medium containing mycelia using a flame-sterilized scalpel or probe. Then spear the agar block of mushroom starter with the probe, lift up the lid of the jar, and drop in the piece of mushroom starter. Close the lid but do not screw it too tight since it is necessary for growing mycelia to breathe. To enhance the rate of mycelial growth, very soon after the jar is inoculated the lid can be screwed tight and the jar shaken to bring the piece of mushroom starter into contact with more of the rice-cake surface. Then loosen the lid before setting the jar in place to incubate. In about four weeks mushrooms will start to grow. Sometimes they commence after only three weeks, but they may frequently take up to six weeks to appear. This depends a lot on the strain and room temperature.

POLLOCK SH
Magic Mushroom Cultivation (2)
Magic Mushroom Cultivation. Herbal Medicine Research Foundation. ISBN 0-930074-01-7
The mycelia can be grown in the dark but light is needed when it is time for the fungus to make mushrooms. As little as five minutes twice a day from an overhead incandescent light in a closet can be sufficient to initiate mushroom formation. But much better crops seem to come when fluorescent 'grow lights' are used for longer periods during the day. When mushrooms are growing, the lid of each jar should be very loose since much condensation occurs as the mushrooms breathe. Some growers remove the lids completely at this time or replace the domes with a double layer of paper towels. The towels can be secured in place with the lid bands and the jars may be set near a window for natural light. Paper towel tops should be sprayed with water at least once a day to help maintain a humid environment. As the rice-cake dries, fruition is promoted. But if the dome is left very loosely in place, fruiting continues much longer. Sometimes fruiting occurs for three months or more! Mushrooms will keep appearing after harvesting of previous crops. To harvest the magic mushrooms, a fancier can reach in through the mouth of the jar and pull them out. It is best to grasp each new mushroom near the bottom of the stem and give it a twist. If the mushroom cap is tugged, it might just break off from the stem. Alternatively, a long knife may be used to cut the mushrooms at the bottom of the stem. Still another method is to turn the jar facing down so that the cake will fall near the orifice. This makes it easier to grasp the mushrooms. Sometimes it is advantageous after a second or third harvest to flip the cake over in the jar before putting the lid back on. This maneuver often promotes a luxuriant fruiting from the newly exposed rice-cake surface. When the cakes have dried out too much for mushrooms to appear, they can be squirted with water from a spray bottle to induce another fruiting or better yet used as spawn for a mushroom garden on compost. If there is absolutely no sign of contamination, the cakes themselves may be fried or broken up and cooked in mushroom soup or other cuisine for a psychedelic experience. One cake is usually sufficient for two to four enthusiasts. The rice-cake technique is very efficient. A 14 ounce package of brown rice can be obtained often for less than fifty cents and is enough for seven quart jars. When the cakes have completely become covered by mycelia, small pieces can be cut out with a sterilized scalpel or probe and transferred to newly prepared rice-cakes in other jars. This will not interfere significantly with mushroom production and will insure a continuing supply of magic San Isidro mushrooms. San Isidro [Psilocybe cubensis] is the only species that has been observed so far to make mushrooms on rice-cakes. Rice-cake medium nevertheless can be used to grow mycelia or other Psilocybe species besides cubensis. Psilocybe cyanescens and subaeruginascens mycelia thrive on brown rice, whereas baeocystis, caerulescens, semilanceata, stuntzii, subaeruginosa, and zapotecorum mycelia spread more slowly on this medium. Brown rice also supports growth of Panaeolus mycelia. With further experimentation, especially with temperature regulation, modifications of the rice-cake technique may render it useful for obtaining fruit from various magic mushroom species.

Psilocybe Cubensis Compost Mushroomkit- 7 page Homestead Company instructions for growing sacred mushrooms. circa early 1990's. [box 5m]

STAMETS, PAUL; CHILTON, J S
Growing Parameters for Psilocybe cubensis (1)
The Mushroom Cultivator: A Practical Guide to Growing Mushrooms at Home; Agarikon Press, Olympia WA; Chapter IX, p 196-203
Growing Parameters for Psilocybe Cubensis
SPECIES: Psilocybe cubensis (Earle) Singer
= Stropharia cubensis Earle
= Stropharia cyanescens Murr
= Stroparia caerulescens (Pat.) Sing
= Naematoloma caerulescens Pat
= Hypholoma caerulescens (Pat.) Sacc. & Trott
STRAINS: Strains of Psilocybe cubensis are available from private and commercial stocks. The American Type Culture Collection, which sells cultures to educational organizations and research facilities, has stock cultures of several wild strains. Note that the strains listed below are only some of those that are presently circulating. There are many more. Some strains may originate from the same region but have features not in agreement with those described here
Amazonian: Medium to large mushrooms on rye grain; thick whitish stems; tenaciously attached to the casing
Ecuadorian: Medium sized mushrooms on rye grain; hemispheric caps; abundant primordia former; high yielding on compost; thin whitish stems; easily picked
Matias Romero: Medium to large mushrooms on rye grain; early fruiter; thick whitish stems and tenaciously attached
Misantla: Medium sized mushrooms on rye grain; thin yellowish stems; tall standing and easily picked
Palenque: Large mushrooms on rye grain; high yielding; and easily picked
COMMON NAMES: San Isidro; Cubensis
GREEK ROOT: Psilocybe comes from the Greek root 'psilos' meaning bald head and cubensis, a name Earle assigned to this mushroom because it was first recognized as a new species from specimens collected in Cuba.

STAMETS, PAUL; CHILTON, J S
Growing Parameters for Psilocybe cubensis (2)
The Mushroom Cultivator: A Practical Guide to Growing Mushrooms at Home; Agarikon Press, Olympia WA; Chapter IX, p 196-203
GENERAL DESCRIPTION: A medium to large size mushroom having a cap that becomes convex to plane in age and is usually pigmented chestnut brown to deep yellowish or golden brown. The cap surface is finely fibrillose, sometimes covered with scattered, fugacious, cottony scales that soon disappear. The partial veil is membranous, well developed and typically leaving a persistent annulus on the upper regions of the stem. The stem is often longitudinally striate, powdered above the annulus and often covered with dense fibrils below. Flesh bruising bluish or bluish green Its spores purplish brown in mass.
NATURAL HABITAT: Naturally found in horse or cow pastures, in dung or in soil enriched with manure. Psilocybe cubensis is a widely distributed species that is found throughout tropical and subtropical zones of the world and is common in the pasturelands of the gulf coast of the southern United States and eastern Mexico.

STAMETS, PAUL; CHILTON, J S
Growing Parameters for Psilocybe cubensis (4)
The Mushroom Cultivator: A Practical Guide to Growing Mushrooms at Home; Agarikon Press, Olympia WA; Chapter IX, p 196-203
Type of Casing: After fully run, cover with the standard casing whose preparation is described in Chapter VII. Layer to a depth of 1-2 inches The casing should be balanced to an initial pH of 6.8-7.2